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ORIGINAL ARTICLE
Year : 2020  |  Volume : 4  |  Issue : 2  |  Page : 159-165

Effect of dexamethasone versus propranolol on surgically induced endometriosis in an experimental animal model


1 Department of Obstetrics and Gynecology, Faculty of Medicine, Al Azhar University (Girls), Cairo, Egypt
2 Department of Experimental Surgery, Medical Research Centre, Ain Shams University, Cairo, Egypt

Correspondence Address:
Naglaa M.S Emsalem

Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/sjamf.sjamf_17_20

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Background Endometriosis is a disease of adolescents and reproductive-aged women characterized by the presence of endometrial tissue outside the uterine cavity and commonly associated with chronic pelvic pain and infertility. The mechanisms responsible for its pathogenesis and progression remain poorly understood. It is well established that endometriosis grows and regresses in estrogen-dependent fashion, and the diseases can be effectively cured by definitive surgery. However, prolonged medical therapy may be needed in most of the cases. Objective The objective of this study is to evaluate the effects of dexamethasone versus propranolol in rats with surgically induced endometriosis on vascular endothelial growth factor (VEGF) as a marker in the implanted tissue. Materials and methods The study was conducted in the Animal Research Unit of Medical Research Center of Ain Shams University. All procedures were performed from May 2018 to September 2018, in compliance with the international guidelines for care and use of experimental animals, on 100 female Wistar albino rats 90 days old, which were randomly divided into three groups: first group was treated with dexamethasone (n=40), and second group was treated with propranolol (n=40), and third group was a control group (n=20). They were used to create a model for the experimental induction of endometriosis and evaluation of therapeutic effect of dexamethasone versus propranolol. Results In the first group after treatment with dexamethasone, there was a highly statistically significant decrease in size of implant tissue (P<0.001). Moreover, it was found regarding the histopathological finding that there was massive necrosis associated with hyalinization of stroma and complete absence of the vascularity. In addition, it was found regarding immunochemical assay (VEGF) that there was mild amount of VEGF (25–50) in the peritoneal tissue of the endometriotic implant. In the second group after treatment with propranolol, there was higher statistical decrease in implant size (P<0.001). It was found there was diffuse necrosis of the glandular structure and hyalinization of the stroma with absence of vascularity and mild amount of VEGF (25–50) in the peritoneal tissue of the endometriotic implant and absence inflammatory cells. However, in the control group, there was a higher statistical increase in implant size (P<0.001). It was found there was no change in endometrial gland and stroma, with severe amount of VEGF (75–100) and inflammatory cells. Conclusion Both propranolol and dexamethasone efficiently reduced the size of endometriotic implant in conditions of surgical-induced endometriosis in rats. This is through restriction of angiogenesis through VEGF, which was utilized as a marker. So, it can be deemed that the drugs could be promoted in conditions of endometriosis in humans, but they require more research studies for assessment in humans.


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